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Research/Technical Note |

Antioxidant and Anti-Inflammatory Activities of the Strychnos spinosa Seeds’ Citric Acid Esters Solution

The Strychnos spinosa seeds contains eriocitrin and neoericitrin which were potential antioxidant molecules. In addition, it contains ursolic acid, lupeol, teraxerol and β-sitosterol which were potential anti-inflammatory molecules. These molecules were extracted from the Strychnos spinosa seeds by esterification with citric acid moles as described in the bibliography in order to synthesize the citric acid ester of Strychnos spinosa seeds. Thus, in this manuscript, the potentiality of this ester solution to be an antioxidant and anti-inflammatory was tested using the spectrophotometry UV-visible method at the wavelength respectively λ=517[nm] and λ=550[nm] according to procedures described in this manuscript. For all tests, a principal-source solution was prepared using the citric acid ester of Strychnos spinosa seeds solution and appropriate solvents; thus from this solution was prepared the solutions at different concentrations to be tested. It was noticed that the concentration ratio between the active molecules and the molecules reacting with these active molecules during the antioxidant and anti-inflammatory tests influenced the quality of the tests. Thus, it was deduced that a low ratio allowed to a better quality results during the spectrophotometer UV-visible antioxidant and anti-inflammatory tests. In consequence, the quantities of the active molecules in the citric acid ester of Strychnos spinosa seeds necessary to the fifty percent diminution of the reacting or formed chemical molecules according to the tests respectively antioxidant tests or anti-inflammatory tests noted Ic-50 were deduced and allowed to evaluate and to compare their activities.

Strychnos spinosa’s Seeds, Flavonoids, Steroids, Antioxidant, Anti-Inflammatory, Ic-50

APA Style

Rabeharitsara, A. T., Raharimalala, M. F., Tsiresy, D. A. F., Robijaona, B., Pierre, R., et al. (2023). Antioxidant and Anti-Inflammatory Activities of the Strychnos spinosa Seeds’ Citric Acid Esters Solution. American Journal of Applied Chemistry, 11(6), 137-145.

ACS Style

Rabeharitsara, A. T.; Raharimalala, M. F.; Tsiresy, D. A. F.; Robijaona, B.; Pierre, R., et al. Antioxidant and Anti-Inflammatory Activities of the Strychnos spinosa Seeds’ Citric Acid Esters Solution. Am. J. Appl. Chem. 2023, 11(6), 137-145. doi: 10.11648/j.ajac.20231106.11

AMA Style

Rabeharitsara AT, Raharimalala MF, Tsiresy DAF, Robijaona B, Pierre R, et al. Antioxidant and Anti-Inflammatory Activities of the Strychnos spinosa Seeds’ Citric Acid Esters Solution. Am J Appl Chem. 2023;11(6):137-145. doi: 10.11648/j.ajac.20231106.11

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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