Volume 4, Issue 3, June 2016, Page: 71-76
First Report on Chemical Composition and Antimicrobial Activity of Artabotrys velutinus Scott-Elliot Extracts Against Some Clinical Strains in Benin
Mahudro Yovo, Laboratory of Study and Research in Applied Chemistry (LERCA), Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Republic of Benin
Guy Alain Alitonou, Laboratory of Study and Research in Applied Chemistry (LERCA), Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Republic of Benin
Hounnankpon Yedomonhan, National Herbarium of Benin, University of Abomey-Calavi, Abomey-Calavi, Republic of Benin
Fidele Tchobo, Laboratory of Study and Research in Applied Chemistry (LERCA), Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Republic of Benin
Oronce Dedome, Laboratory of Study and Research in Applied Chemistry (LERCA), Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Republic of Benin
Philippe Sessou, Laboratory of Study and Research in Applied Chemistry (LERCA), Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Republic of Benin; Laboratory for Research in Applied Biology (LARBA), University of Abomey-Calavi, Abomey-Calavi, Republic of Benin
Félicien Avlessi, Laboratory of Study and Research in Applied Chemistry (LERCA), Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Republic of Benin
Chantal Menut, Institute of Biomolecules Max Mousseron, Team "Glyco and Nanocarriers for Therapeutic Targeting", Faculty of Pharmacy, Montpellier, France
Dominique Sohounhloué, Laboratory of Study and Research in Applied Chemistry (LERCA), Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Republic of Benin
Received: Mar. 24, 2016;       Accepted: Apr. 5, 2016;       Published: Apr. 27, 2016
DOI: 10.11648/j.ajac.20160403.11      View  3031      Downloads  102
Abstract
This work aims to study the chemical composition and to evaluate the antimicrobial activity, for the first time, of essential oil and non-volatile extracts of Artabotrys velutinus against Klebsiella pneumoniae 818E, Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25933, three clinical strains of reference. The chemical analysis of essential oil of Artabotrys velutinus by GC and GC-MS showed that this oil rich in aromatic components (62.6%) and sesquiterpene hydrocarbons (29.9%) contains 30 compounds representing 98.9% of the oil. The major components of the essential oil were benzyl benzoate (61.2%) also called ascabiol with and E-β-caryophyllene (9.1%). The phytochemical screening of leaves powder of Artabotrys velutinus revealed the presence of saponins, catechin tannins, mucilages, flavonoids, alkaloid, anthocyanins, leuco-anthocyanins, reducing compounds, sterols and terpenes. The in vitro antibacterial activity of the extracts by agar diffusion method showed that only the ethanolic extract of the plant was more effective against E. coli with the highest inhibition zone of 13 mm at 100mg/mL and Minimal Inhibitory Concentration equal to 50 mg/mL. However, the activity of ethanolic extract of this plant was less active than those of reference antibiotics chloramphenicol and gentamycin which were very effective against the strains tested. In sum, essential oil of Artabotrys velutinus and its hydroethanolic extract present weakness antimicrobial activity contrary to its ethanolic extract which possesses moderate activity against clinical strains tested. This study suggests the used of ethanolic extract of Artabotrys velutinus in combination with others active extracts to fight against E. coli.
Keywords
Artabotrys velutinus, Essential Oil, Non-volatile Extract, Clinical Strains
To cite this article
Mahudro Yovo, Guy Alain Alitonou, Hounnankpon Yedomonhan, Fidele Tchobo, Oronce Dedome, Philippe Sessou, Félicien Avlessi, Chantal Menut, Dominique Sohounhloué, First Report on Chemical Composition and Antimicrobial Activity of Artabotrys velutinus Scott-Elliot Extracts Against Some Clinical Strains in Benin, American Journal of Applied Chemistry. Vol. 4, No. 3, 2016, pp. 71-76. doi: 10.11648/j.ajac.20160403.11
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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